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Multiplex IHC Detection Kits

Superior MULTIVIEW® IHC kits for efficient detection of multiple antigens in a single tissue sample.

  • Biotin-free nanopolymer-based system for superior multiplex antigen detection
  • Complete kit includes HIGHDEF® chromogens for sensitive, crisp 2-color development
  • Preserves precious tissue while reducing reagent and labor costs
  • Expandable with additional chromogens (sold separately)

Kits Include:

  1. Peroxidase Block
  2. POLYVIEW® HRP & AP Detection Reagents
  3. HIGHDEF®Stable DAB Plus Chromogen & Buffer
  4. HIGHDEFRed Chromogen & Buffer
  5. Mixing bottles

Formalin fixed paraffin embedded human tonsil double stained using MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit (Prod. no. ADI-950-100). Primary rabbit anti-Ki67 antibody was visualized with HIGHDEF® red IHC chromogen (AP) (Prod. no. ADI-950-140), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP) (Prod. no. ADI-950-170, sold separately), producing a distinct golden yellow color contrasting with the bright red.

 

MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit (Brown/Green) 
Superior multiplex IHC kit for  efficient detection of multiple antigens in a single tissue sample.
IHC | Print as PDF
 
ENZ-KIT181-0150 150 tests 751.00 USD
Do you need bulk/larger quantities?
 

MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit 
Superior multiplex IHC kit for efficient detection of multiple antigens in a single tissue sample.
IHC | Print as PDF
 
ADI-950-100-0001 100 tests 515.00 USD
Do you need bulk/larger quantities?
 

Learn how others have used Enzo’s Multiplex
for their studies!

Tumor Phenotyping and Immune Cell Regulation using Multiplex IHC

Made in collaboration with Histalim, Montpellier, France



The main objective of this study was to validate the use of the MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC kit and the HIGHDEF® IHC chromogens to detect multiple markers on human tissue sections. To that effect, two panels composed of four markers each were chosen. The first panel includes phenotypic markers commonly used in the diagnosis of lung cancer (CK5/6, Napsin A, p63, and TTF-1) while the second panel focusses on the characterization of the immune cell response and its regulation (CD3, CD8, PD-1, and PD-L1). The intensity, sensitivity, and specificity obtained with both panels demonstrated the capacity of Enzo’s detection reagents and chromogens in multiplex protocols.

Three-color IHC Staining On Free-floating Sections From Human Brain

Made in collaboration with Histology platform, Brain and Spine Institute, Hôpital Pitié Salpêtrière, Paris, France



The main objective of this study was to validate the use of the MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC Kit and the HIGHDEF ® IHC chromogens to detect multiple staining on human free-floating brain sections. The intensity, sensitivity, and specificity obtained for all staining demonstrate the capacity of the Enzo Life Sciences kit and their chromogen to achieve multiplexing IHC on free-floating tissue sections.



Characterization of Immune Cell Infiltrates in Human Cardiac Allograft Vasculopathy Using MULTIVIEW® IHC Kit

Made in collaboration with Department of Medicine, Columbia University Medical Center, NY, USA



Immunohistochemistry (IHC) is an important diagnostic, prognostic, and research tool to analyze tissue of different origins and different species. However, IHC reagents are not always easily adapted to multiplex staining methods. This application note demonstrates the successful use of Enzo Life Sciences’ MULTIVIEW® (mouse-HRP/rabbit-AP) IHC kit to observe and characterize immune cell infiltrates in human cardiac allograft vasculopathy.

 

Expand your multiplex analysis!

Enzo’s broadest (and expanding) palette of IHC stain colors to choose from, enables you to achieve ultimate flexibility in planning your multiplex Immunohistochemistry protocol.
 
  • Widest panel of color choices
  • Unique yellow, black and blue HRP chromogen
  • Increased flexibility with HRP & AP options
  • High definition color development
IHC picture of formalin-fixed paraffin-embedded human tonsil

Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) ( brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) ( red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (yellow), and methyl green nuclear counterstain.

 

Featured Literature

Brochures
Immunohistochemistry - Complete solutions for IHC analysis
Immunohistochemistry - Complete solutions for IHC analysis
Download as PDF
Catalogs
Antibodies for IHC
Antibodies for IHC
Download as PDF
request the selected literature
All new literature pieces

 

Need help improving your IHC protocols?

Learn more from our tech support team notes!

Do you want to know more about IHC?

Download our IHC E-book

Enzo offers research kits, biochemicals and biologicals backed by over 40 years of expertise. As Scientists Enabling Healthcare™, we realize the value in providing relevant information to our customers working in the fields of life sciences, drug development and clinical research. We are happy to provide simple but useful tips for improving daily tasks as well as the overall quality of your research.
With this in mind, here is an e-book that answers important questions such as:

  • Top 10 Tips for Successful Immunohistochemistry
  • Choosing your Fixation, Embedding, and Antigen Retrieval Method for Successful IHC
  • Controlling Unspecific Background with Blocking
  • Selecting and Optimizing Primary Antibodies for IHC
  • How to Select a Secondary Antibody
  • Various Detection Methods for IHC
  • Choosing the Right Reporter Enzyme and Chromogen
  • Multiplexing Chromogenic IHC
  • Common Counterstains for IHC
  • Useful Controls for Immunohistochemistry
  • IHC Art Gallery
  • Immunohistochemistry Troubleshooting Guide

 IHC E-book

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